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triton in pbs pbst  (Thermo Fisher)


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    Thermo Fisher triton in pbs pbst
    Triton In Pbs Pbst, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/buffer+pbst/Phosphate-buffered+saline+(PBS%2C+10X)%2C+with+Triton%26mid%3Br+X-100/pm42349735-148-20-17
    Average 94 stars, based on 1 article reviews
    triton in pbs pbst - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Blocking Assay:

    Article Title: Suppression of Glucosylceramide Synthase Reverses Drug Resistance in Cancer Cells Harboring Homozygous p53 Mutants
    Article Snippet: Equal amounts of proteins (50 μg/lane) were resolved by using 4–12% gradient SDS-PAGE (Life Technologies). .. The nitrocellulose-membrane blots transferred were blocked in the StartingBlock Blocking buffer (proprietary protein formulation in 0.05% Tween-20, 20 mM phosphate-buffered saline, pH 7.5 (PBST), Thermo Fisher Scientific), and then incubated with each one of the primary antibodies (1:1000 or 1:2000 dilution) at 4 °C overnight. .. These blots were incubated with corresponding horseradish peroxidase–conjugated secondary antibodies (1:5000 dilution) and detected using SuperSignal West Femto substrate (Thermo Fisher Scientific) and ChemiDoc MP imaging system (Bio-Rad, Hercules, CA, USA).

    Article Title: Suppression of Glucosylceramide Synthase Reverses Drug Resistance in Cancer Cells Harboring Homozygous p53 Mutants.
    Article Snippet: Briefly, cells or tissue homogenates were lysed in NP40 cell lysis buffer containing protease inhibitors (Biosource, Camarillo, CA, USA) to extract total cellular proteins following these treatments. https://doi.org/10.3390/ijms27073237 Equal amounts of proteins (50 μg/lane) were resolved by using 4–12% gradient SDS-PAGE (Life Technologies). .. The nitrocellulose-membrane blots transferred were blocked in the StartingBlock Blocking buffer (proprietary protein formulation in 0.05% Tween-20, 20 mM phosphate-buffered saline, pH 7.5 (PBST), Thermo Fisher Scientific), and then incubated with each one of the primary antibodies (1:1000 or 1:2000 dilution) at 4 ◦C overnight. .. These blots were incubated with corresponding horseradish peroxidase–conjugated secondary antibodies (1:5000 dilution) and detected using SuperSignal West Femto substrate (Thermo Fisher Scientific) and ChemiDoc MP imaging system (Bio-Rad, Hercules, CA, USA).

    Formulation:

    Article Title: Suppression of Glucosylceramide Synthase Reverses Drug Resistance in Cancer Cells Harboring Homozygous p53 Mutants
    Article Snippet: Equal amounts of proteins (50 μg/lane) were resolved by using 4–12% gradient SDS-PAGE (Life Technologies). .. The nitrocellulose-membrane blots transferred were blocked in the StartingBlock Blocking buffer (proprietary protein formulation in 0.05% Tween-20, 20 mM phosphate-buffered saline, pH 7.5 (PBST), Thermo Fisher Scientific), and then incubated with each one of the primary antibodies (1:1000 or 1:2000 dilution) at 4 °C overnight. .. These blots were incubated with corresponding horseradish peroxidase–conjugated secondary antibodies (1:5000 dilution) and detected using SuperSignal West Femto substrate (Thermo Fisher Scientific) and ChemiDoc MP imaging system (Bio-Rad, Hercules, CA, USA).

    Article Title: Suppression of Glucosylceramide Synthase Reverses Drug Resistance in Cancer Cells Harboring Homozygous p53 Mutants.
    Article Snippet: Briefly, cells or tissue homogenates were lysed in NP40 cell lysis buffer containing protease inhibitors (Biosource, Camarillo, CA, USA) to extract total cellular proteins following these treatments. https://doi.org/10.3390/ijms27073237 Equal amounts of proteins (50 μg/lane) were resolved by using 4–12% gradient SDS-PAGE (Life Technologies). .. The nitrocellulose-membrane blots transferred were blocked in the StartingBlock Blocking buffer (proprietary protein formulation in 0.05% Tween-20, 20 mM phosphate-buffered saline, pH 7.5 (PBST), Thermo Fisher Scientific), and then incubated with each one of the primary antibodies (1:1000 or 1:2000 dilution) at 4 ◦C overnight. .. These blots were incubated with corresponding horseradish peroxidase–conjugated secondary antibodies (1:5000 dilution) and detected using SuperSignal West Femto substrate (Thermo Fisher Scientific) and ChemiDoc MP imaging system (Bio-Rad, Hercules, CA, USA).

    Saline:

    Article Title: Suppression of Glucosylceramide Synthase Reverses Drug Resistance in Cancer Cells Harboring Homozygous p53 Mutants
    Article Snippet: Equal amounts of proteins (50 μg/lane) were resolved by using 4–12% gradient SDS-PAGE (Life Technologies). .. The nitrocellulose-membrane blots transferred were blocked in the StartingBlock Blocking buffer (proprietary protein formulation in 0.05% Tween-20, 20 mM phosphate-buffered saline, pH 7.5 (PBST), Thermo Fisher Scientific), and then incubated with each one of the primary antibodies (1:1000 or 1:2000 dilution) at 4 °C overnight. .. These blots were incubated with corresponding horseradish peroxidase–conjugated secondary antibodies (1:5000 dilution) and detected using SuperSignal West Femto substrate (Thermo Fisher Scientific) and ChemiDoc MP imaging system (Bio-Rad, Hercules, CA, USA).

    Article Title: Suppression of Glucosylceramide Synthase Reverses Drug Resistance in Cancer Cells Harboring Homozygous p53 Mutants.
    Article Snippet: Briefly, cells or tissue homogenates were lysed in NP40 cell lysis buffer containing protease inhibitors (Biosource, Camarillo, CA, USA) to extract total cellular proteins following these treatments. https://doi.org/10.3390/ijms27073237 Equal amounts of proteins (50 μg/lane) were resolved by using 4–12% gradient SDS-PAGE (Life Technologies). .. The nitrocellulose-membrane blots transferred were blocked in the StartingBlock Blocking buffer (proprietary protein formulation in 0.05% Tween-20, 20 mM phosphate-buffered saline, pH 7.5 (PBST), Thermo Fisher Scientific), and then incubated with each one of the primary antibodies (1:1000 or 1:2000 dilution) at 4 ◦C overnight. .. These blots were incubated with corresponding horseradish peroxidase–conjugated secondary antibodies (1:5000 dilution) and detected using SuperSignal West Femto substrate (Thermo Fisher Scientific) and ChemiDoc MP imaging system (Bio-Rad, Hercules, CA, USA).

    Article Title: A Modular Design of a Cholera Toxin B Subunit-Scaffolded Sub-Virion Nanoparticle Vaccine Against West Nile Virus.
    Article Snippet: Following this, 100 μL of the GM1 solution was added to each well of an immunoassay plate (Thermo Fisher Scientific; cat no. 439454), followed by overnight incubation. .. After washing the plate three times with phosphate- buffered saline with Tween- 20 (PBST) (PBS, Gibco; 0.05% Tween- 20, Merck, Germany), each well was blocked with 200 μL of 1% bovine serum albumin (BSA) for 2 h at room temperature (RT). ..

    Incubation:

    Article Title: Suppression of Glucosylceramide Synthase Reverses Drug Resistance in Cancer Cells Harboring Homozygous p53 Mutants
    Article Snippet: Equal amounts of proteins (50 μg/lane) were resolved by using 4–12% gradient SDS-PAGE (Life Technologies). .. The nitrocellulose-membrane blots transferred were blocked in the StartingBlock Blocking buffer (proprietary protein formulation in 0.05% Tween-20, 20 mM phosphate-buffered saline, pH 7.5 (PBST), Thermo Fisher Scientific), and then incubated with each one of the primary antibodies (1:1000 or 1:2000 dilution) at 4 °C overnight. .. These blots were incubated with corresponding horseradish peroxidase–conjugated secondary antibodies (1:5000 dilution) and detected using SuperSignal West Femto substrate (Thermo Fisher Scientific) and ChemiDoc MP imaging system (Bio-Rad, Hercules, CA, USA).

    Article Title: Ethanol Alters the Neurexin Landscape in Human Neuroblastoma Cells.
    Article Snippet: .. Following ethanol exposure as described above, Undiff and Diff treated cells were fixed in 4% paraformaldehyde (PFA) for 10 min at room temperature, permeabilized with PBS containing 0.1% Tween- 20 for 10 min and blocked with 10% donkey serum in PBST (0.05% Tween- 20) for 1 h. Primary antibodies were applied at the following dilutions: 1:800 PV (catalog no.: PA5- 143579, Invitrogen, Rockford, IL, USA), 1:100 Beta 3 tubulin (TUJ1), 1:800 microtubule- associated protein 2 (MAP2), 1:50 NLGN2, 1:50 NRXN1, and 1:50 NRXN2, 1:400 NRXN3β (catalog no.: A5- 101708, Invitrogen, Rockford, IL, USA) and 1:30 NRXN3α (catalog no.: AF5269, R&D Systems, Minneapolis, MN, USA) in PBS containing 0.05% Tween- 20 and 5% donkey serum, and incubated overnight at 4°C. ..

    Article Title: Dynamic polyadenylation safeguards developmental trajectories during paused embryogenesis
    Article Snippet: .. In brief, samples were blocked in NCS-PBST (10% fetal bovine serum, 1% DMSO, 0.1% Triton X-100 in PBS) for 2 hours, incubated overnight in primary antibodies in NCS-PBSTx rocking at 4°C, and then incubated overnight in secondary antibodies (Invitrogen, Al 1001 and A21245, 1 :500) in NCS-PBSTx rocking at 4°C with either SYTOX green (Invitrogen, S7020, 1 :20,000) or DAPI (Invitrogen, D21490,1:10,000). .. To aid imaging efficiency and accuracy, embryos were flat-mounted after staining for pSmadl/5/9 (Cell Signaling, 13820.

    Article Title: Ethanol Alters the Neurexin Landscape in Human Neuroblastoma Cells
    Article Snippet: .. Following ethanol exposure as described above, Undiff and Diff treated cells were fixed in 4% paraformaldehyde (PFA) for 10 min at room temperature, permeabilized with PBS containing 0.1% Tween‐20 for 10 min and blocked with 10% donkey serum in PBST (0.05% Tween‐20) for 1 h. Primary antibodies were applied at the following dilutions: 1:800 PV (catalog no.: PA5‐143579, Invitrogen, Rockford, IL, USA), 1:100 Beta 3 tubulin (TUJ1), 1:800 microtubule‐associated protein 2 (MAP2), 1:50 NLGN2, 1:50 NRXN1, and 1:50 NRXN2, 1:400 NRXN3β (catalog no.: A5‐101708, Invitrogen, Rockford, IL, USA) and 1:30 NRXN3α (catalog no.: AF5269, R&D Systems, Minneapolis, MN, USA) in PBS containing 0.05% Tween‐20 and 5% donkey serum, and incubated overnight at 4°C. ..

    Article Title: Inhibition of host N-myristoylation compromises the infectivity of SARS-CoV-2 due to Golgi-bypassing egress.
    Article Snippet: Membranes were then incubated with primary antibodies overnight (4°C) using SARS-CoV-2 (2019-nCoV) Spike S2 Antibody, Rabbit PAb (Sino Biological Inc, Cat. no. 40592- T62; 1:1000 dilution), SARS-CoV-2 (2019-nCoV) Spike S2 Antibody, Rabbit PAb (Sino Biological Inc, Cat. no. 40590-T62; 1:1000 dilution), SARS-CoV-2 membrane (M) antibody (R&D Systems, Cat no. MAB10690; 1:1000 dilution), rabbit anti-N protein of SARS-CoV-2 nucleocapsid (1:2000 dilution; gift from Dr. Jussi Hepojoki), anti-NMT1 rabbit IgG polyclonal antibody (Proteintech, Cat. no. 11546-1-AP; 1:500 dilution), anti-NMT2 rabbit IgG polyclonal antibody (Invitrogen, Cat. no. PA5-87291; 1:1000 dilution), anti-beta actin mouse IgG monoclonal antibody (Santa Cruz Biotechnology, Cat. no. sc-47778; 1:1000 dilution). .. Following three washes with 0.1% tween-20 in PBS (PBST; Thermo Fisher Scientific, Cat. no. J63314.AP), the membranes were incubated for 1 h in the following secondary antibodies diluted in 0.1% BSA as applicable: IRDye 680LT Donkey anti-Mouse IgG (H + L) (LICORbio, Cat. no. 926-68022; 1:10000 dilution), IRDye 800CW Goat Anti-Mouse IgG (LICORbio, Cat. no. 926-32210; 1:10000 dilution), IRDye 680RD Goat Anti-Rabbit IgG (LICORbio, Cat. no. 926-68071; 1:10000 dilution), and IRDye 800CW Donkey Anti-Rabbit IgG (LICORbio, Cat. no. 926-32213; 1:10000 dilution). .. Imaging was done with Molecular Device Nanos Automated Microscope (Molecular Devices, LLC).

    Article Title: The ATRXt Protein Represses rDNA Transcription While Mirroring ATRX Interactions and Heterochromatin Localization.
    Article Snippet: .. A total of 20 μL of protein A magnetic beads (Thermo Scientific 88845) were rinsed 1X in IP buffer and resuspended in 200 μL of PBST (PBS with 0.1% Tween-20), and 3 μg of antibody or species matched IgG was added to the bead slurry, followed by incubation on a nutating mixer for 1 h at 4 ◦C. ..

    Article Title: Suppression of Glucosylceramide Synthase Reverses Drug Resistance in Cancer Cells Harboring Homozygous p53 Mutants.
    Article Snippet: Briefly, cells or tissue homogenates were lysed in NP40 cell lysis buffer containing protease inhibitors (Biosource, Camarillo, CA, USA) to extract total cellular proteins following these treatments. https://doi.org/10.3390/ijms27073237 Equal amounts of proteins (50 μg/lane) were resolved by using 4–12% gradient SDS-PAGE (Life Technologies). .. The nitrocellulose-membrane blots transferred were blocked in the StartingBlock Blocking buffer (proprietary protein formulation in 0.05% Tween-20, 20 mM phosphate-buffered saline, pH 7.5 (PBST), Thermo Fisher Scientific), and then incubated with each one of the primary antibodies (1:1000 or 1:2000 dilution) at 4 ◦C overnight. .. These blots were incubated with corresponding horseradish peroxidase–conjugated secondary antibodies (1:5000 dilution) and detected using SuperSignal West Femto substrate (Thermo Fisher Scientific) and ChemiDoc MP imaging system (Bio-Rad, Hercules, CA, USA).

    Magnetic Beads:

    Article Title: The ATRXt Protein Represses rDNA Transcription While Mirroring ATRX Interactions and Heterochromatin Localization.
    Article Snippet: .. A total of 20 μL of protein A magnetic beads (Thermo Scientific 88845) were rinsed 1X in IP buffer and resuspended in 200 μL of PBST (PBS with 0.1% Tween-20), and 3 μg of antibody or species matched IgG was added to the bead slurry, followed by incubation on a nutating mixer for 1 h at 4 ◦C. ..



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